Overordnede kursusmål
Microorganisms are unique sources for diverse chemistry that can be
exploited in biotechnology (e.g. as pharmaceuticals) or feared as
contaminants in food or feed (e.g. mycotoxins). The aim of the
course is to give the students a fundamental insight into this
chemical diversity produced by microorganisms and especially how
these are analyzed. The course introduce the various types of
natural products (secondary metabolism), and analytical strategies
to profile, characterize and quantitate such metabolites also in
the perspective of the metabolomics. The emphasis will be on
chemical diversity within important genera such as: Penicillium,
Aspergillus, Fusarium and Streptomyces.
Læringsmål
En studerende, der fuldt ud har opfyldt kursets mål, vil kunne:
- Define and describe the major classes of natural products
(secondary metabolites).
- Present one of the major pathways leading to secondary
metabolites in some details.
- Describe strategies for sample preparation of microbial
extracts (liquid extraction, solid phase extraction, pKa and LogD
considerations).
- Explain the principles of different types of chromatography
(RP, HILIC, IC) and pH considerations.
- Sketch-out an analytical strategy for a given molecule, UV/Vis,
FLD, GC, LC-MS
- Explain the use for GC-MS for different kinds of small
molecules
- Interpret data from mass spectrometry (spectra and
chromatograms), isotope and adduct patterns.
- Devise data processing approaches for metabolite
profiling.
- Interpretation of simple MS/MS spectra and their use in
dereplication
- Explain the strategies for modern LC-MS/MS target analysis and
being able to handle data analysis
- Being able to make quantitative LC-MS including handling matrix
effects via different startegies
- Within a short timeframe write a report and prepare a
scientific presentation.
Kursusindhold
With onset in microbial metabolites especially fungal, the course
will focus on the following topics: a) the biochemistry of the
chemodiversity in microorganisms including the biosynthesis of
important classes of compounds such as polyketides, terpenoids and
alkaloids; b) concept and strategies of metabolite profiling and
metabolomics, c) the core analytical methodologies used for
detection and analysis of natural products including LC and GC and
detectors e.g. UV (diode array detection), fluorescence,
evaporative light scattering and especially mass spectrometry (MS),
d) data analysis of unit resolution MS and high resolution (TOF) MS
atmospheric pressure ionization spectra; e) data interpretation for
database searching; f) tentative identification of compounds based
on LC-MS data, LogD consideration and ion-exchange sample
preparation strategy, and g) Target analysis of known compounds and
their metabolites based on LC-MS/MS (triple quadrupole data)
including the scan functions of this instrument; h) Solid Phase
Extraction (SPE) purification strategies.
Bemærkninger
Course fee
1) 2,000 DKK for PhD students (The course is free for all PhD
students from Denmark and other EU/EEA countries. However, these
pay a fee for course materials of 2,000 DKK)
2) 2,000 DKK for other participants from academia
3) DKK 12,000 for participants from industry
The course fee includes course materials, lab expenses and meals
during the day.
Sidst opdateret
08. juni, 2017
