Efterår
September 2014
One month
Class contact (lecture) 0 hours
Class contact (practical) 0 hours
Specified learning (textbook 32 hours
Autonomous learning literature)32 hours
Undervisningens placering:
The course is exclusively online. The communication between
teacher and students will be through CampusNet at DTU Portal. The
teaching will be supplemented through webinars at YouTube. There
will be no physical meeting or class attendance. A textbook
(published by the course teacher) will be the main reading
material. The reading will be supplemented with publications from
literature. It is important that all registered students have
access to the Internet and can be reached via
e-mail.
Undervisningsform:
The course is exclusively online. The communication between
teacher and students will be through CampusNet at DTU Portal. The
teaching will be supplemented through webinars at YouTube. There
will be no physical meeting or class attendance. A textbook
(published by the course teacher) will be the main reading
material. The reading will be supplemented with publications from
literature. It is important that all registered students have
access to the Internet and can be reached via e-mail.
Ingen eksamen, Aftales med underviser, Passed/not
passed. In order to pass, the student must have answered 75% (3 out
of 4) of the assignments correctly. This will be judged by the main
teacher and an external censor. There will be no final
examination.
Evalueringsform:
Hjælpemidler:
Bedømmelsesform:
Pointspærring:
Overordnede kursusmål:
The main objective of the course is to provide knowledge in modern
traceability techniques in relation to food safety. Traceability of
microorganisms and their toxins along the entire food production
chain and the use of advanced methods to trace and track these are
essential to ensure the safety/security of the food chain. This
course will review the most important Foodborne pathogens that can
be transmitted through the food chains to humans. It provides a
thorough introduction to advanced molecular methods for detection,
enumeration and characterization. It will present a structured and
detailed description of main pathogens and their specific detection
from the point of sampling through sample pre-treatment, analysis
and data analysis.
The course proposed is part of the BIOTRACER Network to support the
participation of DTU in the EU Research Framework Program Horizon
2020.
Læringsmål:
En studerende, der fuldt ud har opfyldt kursets mål, vil kunne:
Design appropriate sampling methods for different
pathogens
Understand the cons and pros of modern techniques compared to
culture-based methods
Select appropriate diagnostic methods for rapid detection
purposes
Choose the right pre-analytical sample treatment protocol and
test method for each food commodity
Select the right method for the right commodity
Learn about the possibilities and limitations of DNA techniques
for detection and sub-typing purposes.
Learn about Food Safety Objectives and the consequences for
rapid testing
Become familiar with sampling strategy and sample pooling
techniques
Kursusindhold:
The studies will be organized in four following themes:
Theme 1. Critical considerations in setting up rapid methods
(sampling, statistics, validation, etc.).
Theme 2. Current detection and typing methods.
Theme 3. Fresh produce, water and seafood testing for pathogens.
Theme 4. Future of advanced laboratory methods.
Both classical and modern methods for detection of pathogens will
be reviewed in relation to laboratory diagnostics, monitoring,
surveillance and source attribution programs. A major part of the
studies will include sampling strategies for different pathogens,
bottlenecks in pre-analytical sample treatment for rapid tracing,
rapid methods of analysis, enumeration and characterization of
pathogens, and critical evaluation of different methods.
Litteraturhenvisninger:
Text book: Rapid Detection, Characterization and Enumeration of
Foodborne Pathogens (2011). Hoorfar J. (ed.). American Society for
Microbiology. Washington, D.C., USA. ISBN 978-1-55581-542-4.
